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23 February 2009 Continuous-flow PCR using segmented flow and integrating sample preparation
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Continuous-flow PCR has proven to be a powerful method for the amplification of genetic material due to its high speed and the possibility to perform amplicon detection and separation on-chip. A unique possibility of this method is the simultaneous amplification of several samples within a single chip by sample stacking, either having identical samples in several sample plugs separated by e.g. a mineral oil or using different samples in each sample plug. We have demonstrated the viability of sample stacking with a commercially available continuous-flow PCR system with a variety of protocols and samples. Further integration steps like thermal lysis and on-chip lyophilisate storage have been performed, with subsequent successful PCR. Chip modules for DNA extraction either with magnetic beads or membrane filters have been developed.
© (2009) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Holger Becker, Nadine Hlawatsch, Cornelia Carstens, Richard Klemm, and Claudia Gärtner "Continuous-flow PCR using segmented flow and integrating sample preparation", Proc. SPIE 7207, Microfluidics, BioMEMS, and Medical Microsystems VII, 72070J (23 February 2009);

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