DLP hyperspectral imaging for surgical and clinical utility

Karel J. Zuzak; Robert P. Francis; Eleanor F. Wehner; Jack Smith; Maritoni Litorja; David W. Allen; Chad Tracy; Jeffrey Cadeddu; Edward Livingston

doi:10.1117/12.810068

13 February 2009 DLP hyperspectral imaging for surgical and clinical utility

Karel J. Zuzak, Robert P. Francis, Eleanor F. Wehner, Jack Smith, Maritoni Litorja, David W. Allen, Chad Tracy, Jeffrey Cadeddu, Edward Livingston

Author Affiliations +

Proceedings Volume 7210, Emerging Digital Micromirror Device Based Systems and Applications; 721006 (2009) https://doi.org/10.1117/12.810068
Event: SPIE MOEMS-MEMS: Micro- and Nanofabrication, 2009, San Jose, California, United States

Abstract

We describe a novel digital light processing, DLP hyperspectral imaging system for visualizing chemical composition of in vivo tissues during surgical procedures non-invasively and at near video rate. The novelty of the DLP hyperspectral imaging system resides in (1) its ability to conform light to rapidly sweep through a series of preprogrammed spectral illuminations as simple as a set of contiguous bandpasses to any number of complex spectra, and (2) processing the reflected spectroscopic image data using unique supervised and unsupervised chemometric methods that color encode molecular content of tissue at each image detector pixel providing an optical biopsy. Spectral illumination of tissue is accomplished utilizing a DLP® based spectral illuminator incorporating a series of bandpass spectra and measuring the reflectance image with a CCD array detector. Wavelength dependent images are post processed with a multivariate least squares analysis method using known reference spectra of oxy- and deoxyhemoglobin. Alternatively, illuminating with complex reference spectra reduces the number of spectral images required for generating chemically relevant images color encoded for relative percentage of oxyhemoglobin are collected and displayed in real time near-video rate, (3 to 4) frames per second (fps). As a proof of principle application, a kidney of an anesthetized pig was imaged before and after renal vasculature occlusion showing the clamped kidney to be 61% of the unclamped kidney percentage of oxyhemoglobin. Using the "3-Shot" spectral illumination method and gathering data at (3 to 4) fps shows a non-linear exponential de-oxygenation of hemoglobin reaching steady state within 30 seconds post occlusion.

Citation Download Citation

Karel J. Zuzak, Robert P. Francis, Eleanor F. Wehner, Jack Smith, Maritoni Litorja, David W. Allen, Chad Tracy, Jeffrey Cadeddu, and Edward Livingston "DLP hyperspectral imaging for surgical and clinical utility", Proc. SPIE 7210, Emerging Digital Micromirror Device Based Systems and Applications, 721006 (13 February 2009); https://doi.org/10.1117/12.810068

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available