Non-rigid alignment of multi-channel fluorescence microscopy images of live cells for improved classification of subcellular particle motion

Il-Han Kim; Roland Eils; Karl Rohr

doi:10.1117/12.811543

27 February 2009 Non-rigid alignment of multi-channel fluorescence microscopy images of live cells for improved classification of subcellular particle motion

Il-Han Kim, Roland Eils, Karl Rohr

Author Affiliations +

Proceedings Volume 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging; 72620S (2009) https://doi.org/10.1117/12.811543
Event: SPIE Medical Imaging, 2009, Lake Buena Vista (Orlando Area), Florida, United States

Abstract

The observed motion of subcellular particles in fluorescence microscopy image sequences of live cells is generally a superposition of the motion and deformation of the cell and the motion of the particles. Decoupling the two types of movements to enable accurate classification of the particle motion requires the application of registration algorithms. We have developed an intensity-based approach which is based on an optic-flow estimation algorithm for non-rigid registration of multi-channel microscopy image sequences of cell nuclei. First, based on 3D synthetic images we demonstrate that cell nucleus deformations change the observed motion types of particles and that our approach allows to recover the original motion. Second, we have successfully applied our approach to register 2D and 3D real microscopy image sequences. A quantitative comparison with a previous scheme has also been performed.

Citation Download Citation

Il-Han Kim, Roland Eils, and Karl Rohr "Non-rigid alignment of multi-channel fluorescence microscopy images of live cells for improved classification of subcellular particle motion", Proc. SPIE 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging, 72620S (27 February 2009); https://doi.org/10.1117/12.811543

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