Multimodal three-dimensional imaging with isometric high resolution using optical projection tomography

Qin Miao; J. Richard Rahn; Ryland C. Bryant; Christy A. Lancaster; Anna Tourovskaia; Thomas Neumann; Eric J. Seibel; Alan C. Nelson

doi:10.1117/12.813844

27 February 2009 Multimodal three-dimensional imaging with isometric high resolution using optical projection tomography

Qin Miao, J. Richard Rahn, Ryland C. Bryant, Christy A. Lancaster, Anna Tourovskaia, Thomas Neumann, Eric J. Seibel, Alan C. Nelson

Author Affiliations +

Proceedings Volume 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging; 72620V (2009) https://doi.org/10.1117/12.813844
Event: SPIE Medical Imaging, 2009, Lake Buena Vista (Orlando Area), Florida, United States

Abstract

The optical projection tomography microscope (OPTM) is an optical microscope that acquires focus-invariant images from multiple views of single cells. Although the depth of field of the objective is short, it can be extended by scanning the objective's focal plane. This extended depth of field image is similar to a projection in conventional X-ray CT. Samples flow through a microcapillary tube filled with optical gel. Optical distortion is minimized by matching refractive index of optical gel and tube. Multiple projection images are taken by rotating the microcapillary tube with sub-micron mechanical precision. After these pseudoprojection images are further aligned, computed tomography methods are then applied to the images to create a 3D reconstruction with isometric resolution of 0.35 microns. Three-dimensional reconstructed images of fluorescent microspheres and cells are shown.

Citation Download Citation

Qin Miao, J. Richard Rahn, Ryland C. Bryant, Christy A. Lancaster, Anna Tourovskaia, Thomas Neumann, Eric J. Seibel, and Alan C. Nelson "Multimodal three-dimensional imaging with isometric high resolution using optical projection tomography", Proc. SPIE 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging, 72620V (27 February 2009); https://doi.org/10.1117/12.813844

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