Three-dimensional localization of in vivo bioluminescent source based on multispectral imaging

Jinchao Feng; Kebin Jia; Jie Tian; Guorui Yan; Shouping Zhu

doi:10.1117/12.811329

27 February 2009 Three-dimensional localization of in vivo bioluminescent source based on multispectral imaging

Jinchao Feng, Kebin Jia, Jie Tian, Guorui Yan, Shouping Zhu

Author Affiliations +

Proceedings Volume 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging; 72622C (2009) https://doi.org/10.1117/12.811329
Event: SPIE Medical Imaging, 2009, Lake Buena Vista (Orlando Area), Florida, United States

Abstract

Bioluminescence tomography (BLT) is a novel in vivo technique in small animal studies, which can reveal the molecular and cellular information at the whole-body small animal level. At present, there is an increasing interest in multispectral bioluminescence tomography, since multispectral data acquisition could improve the BLT performance significantly. In view to the ill-posedness of BLT problem, we develop an optimal permissible source region strategy to constrain the possible solution of the source by utilizing spectrum character of bioluminescent source. Then a linear system to link the measured data with the unknown light source variables is established by utilizing the optimal permissible region strategy based on adaptive finite element analysis. Furthermore, singular value decomposition analysis is used for data dimensionality reduction and improving computational efficiency in multispectral case. The reconstructed speed and stability benefit from adaptive finite element, the permissible region strategy and singular value decomposition. In the numerical simulation, the heterogeneous phantom experiment has been used to evaluate the performance of the proposed algorithm with the Monte Carlo based synthetic data. The reconstruction results demonstrate the merits and potential of our methodology for localizing bioluminescent source.

Citation Download Citation

Jinchao Feng, Kebin Jia, Jie Tian, Guorui Yan, and Shouping Zhu "Three-dimensional localization of in vivo bioluminescent source based on multispectral imaging", Proc. SPIE 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging, 72622C (27 February 2009); https://doi.org/10.1117/12.811329

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