Microsphere-enhanced label-free high-throughput molecular detection

Cheng Deng; Guoliang Huang; Shukuan Xu; Jing Zhu; Rui Ma; Chao Han; Shengyi Chen; Chen Ma

doi:10.1117/12.823373

6 March 2009 Microsphere-enhanced label-free high-throughput molecular detection

Cheng Deng, Guoliang Huang, Shukuan Xu, Jing Zhu, Rui Ma, Chao Han, Shengyi Chen, Chen Ma

Proceedings Volume 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine; 72800S (2009) https://doi.org/10.1117/12.823373
Event: Photonics and Optoelectronics Meetings, 2008, Wuhan, China

Abstract

In this paper, we developed a microsphere enhanced and label free high throughput molecular detection based on SPRI and microarray chips, and a self-built surface plasmon resonance imaging instrument was set. One label-free protein chip forming a 7× probe array was designed and fabricated using a commercial microarray robot spotter on chemical modified gold-coated glass slide. Antibody molecules were successfully detected in label-free and high-throughput method by using this chip. The detection signals on the chip was successfully enhanced by using microspheres with a diameter of 1 μm.

Citation Download Citation

Cheng Deng, Guoliang Huang, Shukuan Xu, Jing Zhu, Rui Ma, Chao Han, Shengyi Chen, and Chen Ma "Microsphere-enhanced label-free high-throughput molecular detection", Proc. SPIE 7280, Seventh International Conference on Photonics and Imaging in Biology and Medicine, 72800S (6 March 2009); https://doi.org/10.1117/12.823373

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