Scientific CCD camera caused bias estimates of low-light-level quantitative fluorescence imaging measurement

Zhi-wen Hu; Jian Zhang

doi:10.1117/12.828753

20 May 2009 Scientific CCD camera caused bias estimates of low-light-level quantitative fluorescence imaging measurement

Zhi-wen Hu, Jian Zhang

Author Affiliations +

Proceedings Volume 7283, 4th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment; 72832Z (2009) https://doi.org/10.1117/12.828753
Event: AOMATT 2008 - 4th International Symposium on Advanced Optical Manufacturing, 2008, Chengdu, Chengdu, China

Abstract

Scientific CCD cameras with high sensitivity have been widely used for low-light-level quantitative fluorescence microscopy (QFM) in cell biology. These state-of-the-art scientific CCDs offer several advantages over others, but what's new is not always the best choice for every application, and their specifications don't always tell the whole story. So, many pivotal technologies are poorly elucidated which would cause serious errors in QFM. The present study systematically analyzes theoretical reasons and technical sources of discrepancies occurring in QFM. In order to avoid the errors and achieve the accurate determination, this study proposes solutions and suggests some critical measures in both theoretical and technical aspects. Therefore, this analysis can be a valuable tool in scrutinizing multifarious parameters and clarifying proper usages of a scientific CCD camera, especially in low-light-level quantitative fluorescence imaging measurement.

Citation Download Citation

Zhi-wen Hu and Jian Zhang "Scientific CCD camera caused bias estimates of low-light-level quantitative fluorescence imaging measurement", Proc. SPIE 7283, 4th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 72832Z (20 May 2009); https://doi.org/10.1117/12.828753

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