Lab-on-a-chip PCR in continuous flow: an ultrafast analytical tool for B-agents

Claudia Gärtner; Holger Becker; Thomas Clemens; Richard Klemm; Matthias Klotz; Nadine Hlawatsch

doi:10.1117/12.819429

8 May 2009 Lab-on-a-chip PCR in continuous flow: an ultrafast analytical tool for B-agents

Claudia Gärtner, Holger Becker, Thomas Clemens, Richard Klemm, Matthias Klotz, Nadine Hlawatsch

Proceedings Volume 7304, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X; 73040G (2009) https://doi.org/10.1117/12.819429
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

The overall aim is the realization of a reliable, ultrafast, and portable tool for the identification of B-agents at the point of interest. PCR is the method to be used for the doubtless identification of e.g. bacteria, and viruses. Miniaturization is the way to include the overall analysis process, from sample preparation to detection, on a microtiterplate-sized consumable device and to allow to carry out the analysis without the need for an equipped biological laboratory. The novel PCR concept with constant temperature zones allows also for the instrument to become portable, due to much reduced power consumption since no thermocycling is necessary. This paper focuses on the overall concept to implement the biological reactions for the analysis on-chip, the methods of sample preparation on-chip, and the results of the ultrafast PCR with B-agents on-chip, as well as the basic instrument.

Citation Download Citation

Claudia Gärtner, Holger Becker, Thomas Clemens, Richard Klemm, Matthias Klotz, and Nadine Hlawatsch "Lab-on-a-chip PCR in continuous flow: an ultrafast analytical tool for B-agents", Proc. SPIE 7304, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing X, 73040G (8 May 2009); https://doi.org/10.1117/12.819429

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available