Salivary diagnostics: a new solution for an old problem: breast cancer detection

Charles F. Streckfus

doi:10.1117/12.820117

5 May 2009 Salivary diagnostics: a new solution for an old problem: breast cancer detection

Charles F. Streckfus

Proceedings Volume 7306, Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI; 730608 (2009) https://doi.org/10.1117/12.820117
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

Objective: The objective of this study is to determine if LC/MS/MS based isotopic tagging technique is useful to detect putative breast cancer markers in saliva. Methods: Six pooled (n=10 subjects per pool) stimulated whole saliva specimens from women were analyzed. One pooled specimen was from healthy women, another pooled specimen from women diagnosed with a benign breast tumor and the other four-pooled specimens were from women diagnosed with carcinoma of the breast. The four cancer specimens were staged 0, I, IIa and IIb (lymph node involvement). Isotopically tagging proteins in the tumor groups and comparing them to the healthy control group measured differential expression of proteins. The iTRAQ labels are isobaric and chemically identical, thus labeled peptides from the different pools have identical elution times and masses. The relative concentration of identified proteins in each of the mixtures is determined from the intensities of each of the reporter ions. Results: The results of the salivary analyses yielded approximately 209 proteins in the saliva specimens. These proteins were able to distinguish between the healthy control, the benign and the cancer patients. Additionally, there were proteins able to distinguish between node positive and node negative patients.

Citation Download Citation

Charles F. Streckfus "Salivary diagnostics: a new solution for an old problem: breast cancer detection", Proc. SPIE 7306, Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI, 730608 (5 May 2009); https://doi.org/10.1117/12.820117

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