Fiber optic SERS-based plasmonics nanobiosensing in single living cells

Jonathan P. Scaffidi; Molly K. Gregas; Victoria Seewaldt; Tuan Vo-Dinh

doi:10.1117/12.824453

30 April 2009 Fiber optic SERS-based plasmonics nanobiosensing in single living cells

Jonathan P. Scaffidi, Molly K. Gregas, Victoria Seewaldt, Tuan Vo-Dinh

Proceedings Volume 7312, Advanced Environmental, Chemical, and Biological Sensing Technologies VI; 731205 (2009) https://doi.org/10.1117/12.824453
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

We describe the development of small molecule-sensitive plasmonics-active fiber-optic nanoprobes suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The practical utility of SERS-based fiber-optic nanoprobes is illustrated by measurements of intracellular pH in HMEC- 15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor biologically-relevant small molecules at the single cell level.

Citation Download Citation

Jonathan P. Scaffidi, Molly K. Gregas, Victoria Seewaldt, and Tuan Vo-Dinh "Fiber optic SERS-based plasmonics nanobiosensing in single living cells", Proc. SPIE 7312, Advanced Environmental, Chemical, and Biological Sensing Technologies VI, 731205 (30 April 2009); https://doi.org/10.1117/12.824453

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