Applications of spectral imaging using a tunable laser source

David C. Oertel; Jeffrey T. Grothaus; Curtis Marcott

doi:10.1117/12.818147

28 April 2009 Applications of spectral imaging using a tunable laser source

David C. Oertel, Jeffrey T. Grothaus, Curtis Marcott

Proceedings Volume 7319, Next-Generation Spectroscopic Technologies II; 731906 (2009) https://doi.org/10.1117/12.818147
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

Hyperspectral reflectance imaging in the visible and NIR spectral ranges has considerable utility for revealing spatial and chemical complexity in both biological systems and manufactured products. Conventional imaging systems are based on broad-band illumination in tandem with a spectrometer or tunable filter placed between the sample and the detector. These systems are typically slow (require seconds of integration per wavelength step), and the CW broad-band source can cause significant heating of the sample. An alternative method is to use a tunable, pulsed, high-peak-power (low average power) source coupled with a broad-band detector. This approach offers a reduction in data acquisition time, the inherent ability to stop motion, and data collection at ambient temperature. An integrated system based on a 5- ns pulsed laser tunable from 430 nm to 2150 nm has been used to obtain hyperspectral images in both the visible and NIR spectral ranges. A number of camera/lens options allow for varied spectral bandwidths and the FOV, ranging from 11 × 15 mm² to 15 × 20 cm². An entire hyperspectral image stack can be collected in as little as 20 s. This method, allowing fast, room-temperature data acquisition, has sufficient sensitivity to produce data that can be successfully processed using spectral derivatives and multivariate analysis. We discuss several applications, both in vivo and otherwise, of this alternative approach to visible/NIR hyperspectral imaging.

Citation Download Citation

David C. Oertel, Jeffrey T. Grothaus, and Curtis Marcott "Applications of spectral imaging using a tunable laser source", Proc. SPIE 7319, Next-Generation Spectroscopic Technologies II, 731906 (28 April 2009); https://doi.org/10.1117/12.818147

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