A fluorescent immunoassay for the determination of procalcitonin and C-reactive protein

F. Baldini; L. Bolzoni; A. Giannetti; G. Porro; F. Senesi; C. Trono

doi:10.1117/12.821005

18 May 2009 A fluorescent immunoassay for the determination of procalcitonin and C-reactive protein

F. Baldini, L. Bolzoni, A. Giannetti, G. Porro, F. Senesi, C. Trono

Proceedings Volume 7356, Optical Sensors 2009; 735613 (2009) https://doi.org/10.1117/12.821005
Event: SPIE Optics + Optoelectronics, 2009, Prague, Czech Republic

Abstract

The discrimination of viral and bacterial sepsis is an important issue in intensive care patients. For this purpose, the simultaneous measurements of different analytes such as C-reactive protein (CRP), procalcitonin (PCT), myeloperoxidase, interleukines and neopterin, are necessary. A novel optical platform was designed and realised for the implementation of fluorescence-based immunoassays. The core of the optical platform is a plastic biochip, formed by a series of microchannels each of them devoted to the determination of a single analyte. Sandwich assays for CRP and PCT spiked in serum were performed in order to demonstrate the reliability of a multi-array device.

Citation Download Citation

F. Baldini, L. Bolzoni, A. Giannetti, G. Porro, F. Senesi, and C. Trono "A fluorescent immunoassay for the determination of procalcitonin and C-reactive protein", Proc. SPIE 7356, Optical Sensors 2009, 735613 (18 May 2009); https://doi.org/10.1117/12.821005

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