Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy

Silvia Soria; Franco Quercioli; Raffaella Mercatelli; Federica Bianco; Ilaria Cacciari; Stefano Pelli; Giancarlo Righini

doi:10.1117/12.821199

20 May 2009 Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy

Silvia Soria, Franco Quercioli, Raffaella Mercatelli, Federica Bianco, Ilaria Cacciari, Stefano Pelli, Giancarlo Righini

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Proceedings Volume 7366, Photonic Materials, Devices, and Applications III; 73660R (2009) https://doi.org/10.1117/12.821199
Event: SPIE Europe Microtechnologies for the New Millennium, 2009, Dresden, Germany

Abstract

We report on the application of a simple white light source based on the supercontinuum generation from commercial photonic crystal fibres to confocal fluorescence microscopy and fluorescence lifetime imaging (FLIM) microscopy. The coherent white light can be tuned by varying the wavelength and intensity of the pump, a Ti:Sapphire laser. There are several advantages jn the use of SC sources: spatially coherent white radiation, tuning ranges of approximately 400 nm, high brightness, a robust compact system (potentially all-fibre) and relatively low cost. Being pulsed, SC sources are suitable for FLIM and may be used for multiple excitation.

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Silvia Soria, Franco Quercioli, Raffaella Mercatelli, Federica Bianco, Ilaria Cacciari, Stefano Pelli, and Giancarlo Righini "Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy", Proc. SPIE 7366, Photonic Materials, Devices, and Applications III, 73660R (20 May 2009); https://doi.org/10.1117/12.821199

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KEYWORDS

Confocal microscopy

Fluorescence lifetime imaging

Luminescence

Microscopy

Dispersion

Microscopes

Photonic crystal fibers

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