A membrane-associated FRET sensor for detection of apoptosis

Herbert Schneckenburger; Michael Wagner; Petra Weber; Thomas Bruns; Heiko Steuer; Brigitte Angres

doi:10.1117/12.831570

7 July 2009 A membrane-associated FRET sensor for detection of apoptosis

Herbert Schneckenburger, Michael Wagner, Petra Weber, Thomas Bruns, Heiko Steuer, Brigitte Angres

Proceedings Volume 7368, Clinical and Biomedical Spectroscopy; 73680Q (2009) https://doi.org/10.1117/12.831570
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

A membrane associated caspase sensor based on Förster Resonance Energy Transfer (FRET) between enhanced cyan fluorescent protein (Mem-ECFP) and yellow fluorescent protein (EYFP) is reported. Upon apoptosis a caspase sensitive amino acid peptide linker (DEVD) between these proteins is cleaved, and pronounced changes of fluorescence spectra and lifetimes are observed. Membrane selective detection of fluorescent proteins in cultivated HeLa cervix carcinoma cells is achieved by total internal reflection fluorescence microscopy (TIRFM) with high sensitivity and resolution.

Citation Download Citation

Herbert Schneckenburger, Michael Wagner, Petra Weber, Thomas Bruns, Heiko Steuer, and Brigitte Angres "A membrane-associated FRET sensor for detection of apoptosis", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680Q (7 July 2009); https://doi.org/10.1117/12.831570

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KEYWORDS

Luminescence

Cell death

Fluorescence resonance energy transfer

Glasses

Fluorescent proteins

Sensors

Energy transfer

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