5-ALA/PpIX fluorescence detection of gastrointestinal neoplasia

Ekaterina G. Borisova; Borislav Vladimirov; Ivan Terziev; Radina Ivanova; Latchezar Avramov

doi:10.1117/12.831604

7 July 2009 5-ALA/PpIX fluorescence detection of gastrointestinal neoplasia

Ekaterina G. Borisova, Borislav Vladimirov, Ivan Terziev, Radina Ivanova, Latchezar Avramov

Proceedings Volume 7368, Clinical and Biomedical Spectroscopy; 736824 (2009) https://doi.org/10.1117/12.831604
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

In the recent study delta-ALA/PpIX is used as fluorescent marker for dysplasia and tumor detection in esophagus, stomach and colon. ALA is administered per os six to eight (depending on the lesion location) hours before measurements at dose 20mg/kg weight. High-power light-emitting diode at 405 nm is used as an excitation source. Special opto-mechanical device is built for the LED to use the light guide of standard video-endoscopic system. Through endoscopic instrumental channel a fiber is applied to return information about fluorescence to microspectrometer. The fluorescence detected from tumor sites has very complex spectral origins. It consists of autofluorescence, fluorescence from exogenous fluorophores and re-absorption from the chromophores accumulated in the tissue investigated. Spectral features observed during endoscopic investigations could be distinct as the next regions: 450-630 nm region, where tissue autofluorescence is observed; 630-710 nm region, where fluorescence of PpIX is clearly pronounced; 530-580 nm region, where minima in the autofluorescence signal are observed, related to re-absorption of oxy-hemoglobin in this spectral area. Endogenous and exogenous fluorescence spectra are used to develop simple but effective algorithm, based on dimensionless ratio of the signals at 560 and 635 nm, for differentiation of normal/abnormal gastrointestinal tissues. Very good correlation between fluorescence signals and histology examination of the lesions investigated is achieved.

Citation Download Citation

Ekaterina G. Borisova, Borislav Vladimirov, Ivan Terziev, Radina Ivanova, and Latchezar Avramov "5-ALA/PpIX fluorescence detection of gastrointestinal neoplasia", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 736824 (7 July 2009); https://doi.org/10.1117/12.831604

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