Improving FRET detection in living cells

Ching-Wei Chang; Mei Wu; Sofia D. Merajver M.D.; Mary-Ann Mycek

doi:10.1117/12.831718

1 July 2009 Improving FRET detection in living cells

Ching-Wei Chang, Mei Wu, Sofia D. Merajver M.D., Mary-Ann Mycek

Proceedings Volume 7370, Molecular Imaging II; 737007 (2009) https://doi.org/10.1117/12.831718
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Unambiguous FRET detection in living cells is often challenging. Here we describe how the advantages of fluorescence lifetime sensing with FLIM, fluorophore selection, and critical environmental controls provide better FRET detection.

Citation Download Citation

Ching-Wei Chang, Mei Wu, Sofia D. Merajver M.D., and Mary-Ann Mycek "Improving FRET detection in living cells", Proc. SPIE 7370, Molecular Imaging II, 737007 (1 July 2009); https://doi.org/10.1117/12.831718

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