Light collection from fluorescence-based biochips by holographic diffractive optical elements

Peter Macko; Maurice P. Whelan

doi:10.1117/12.831730

9 July 2009 Light collection from fluorescence-based biochips by holographic diffractive optical elements

Peter Macko, Maurice P. Whelan

Proceedings Volume 7371, Novel Optical Instrumentation for Biomedical Applications IV; 73711E (2009) https://doi.org/10.1117/12.831730
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

A fluorescence-based biochip with an integrated holographic diffractive optical element on its underside is presented. The diffractive element is a thick volume hologram written into a layer of photopolymer recording material. The element acts as a collector of the spatially anisotropic fluorescence light emitted from surface-bound fluorophores and redirects the light to a CCD detector. The holographic lithography setup used to fabricate the diffractive elements is described. The performance of the diffractive elements to enhance the light collection is demonstrated.

Citation Download Citation

Peter Macko and Maurice P. Whelan "Light collection from fluorescence-based biochips by holographic diffractive optical elements", Proc. SPIE 7371, Novel Optical Instrumentation for Biomedical Applications IV, 73711E (9 July 2009); https://doi.org/10.1117/12.831730

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