In vivo in situ en face optical coherence tomography imaging of chick embryos

Michael Leitner; Joana Castanheira; Luís Ferreira; Mónica Ferreira; Isabel Palmeirim; Carla C. Rosa; Adrian Gh. Podoleanu

doi:10.1117/12.831859

13 July 2009 In vivo in situ en face optical coherence tomography imaging of chick embryos

Michael Leitner, Joana Castanheira, Luís Ferreira, Mónica Ferreira, Isabel Palmeirim, Carla C. Rosa, Adrian Gh. Podoleanu

Author Affiliations +

Proceedings Volume 7372, Optical Coherence Tomography and Coherence Techniques IV; 73720Y (2009) https://doi.org/10.1117/12.831859
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Chick embryos are among the most studied species in development biology because they are easily obtained, highly accessible and present a similar development to that of humans. Normally morphological studies are carried out with confocal microscopy, however in-situ imaging is impossible and in- vivo imaging can only be performed with great difficulty. For confocal microscopy the embryo has to be studied outside the egg, what generally also means a short life expectancy of the embryo. Additionally, extracting the embryo of the egg precludes the possibility of studying its development in its natural environment. In this paper it is shown that en-face optical coherence tomography (en-face OCT) is a possible solution to overcome these difficulties allowing for an in-situ and in-vivo study over a timescale of several days. With en-face OCT it is possible to accompany the development of one single embryo over several days and to acquire high resolution and axially resolved images.

Citation Download Citation

Michael Leitner, Joana Castanheira, Luís Ferreira, Mónica Ferreira, Isabel Palmeirim, Carla C. Rosa, and Adrian Gh. Podoleanu "In vivo in situ en face optical coherence tomography imaging of chick embryos", Proc. SPIE 7372, Optical Coherence Tomography and Coherence Techniques IV, 73720Y (13 July 2009); https://doi.org/10.1117/12.831859

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