Synthesis and cellular localization of porphyrinic pigments

Sarah Sareh; Sarah Kong; Lenin Parrales; Anna Jung; Kara Cross; Beate Röder; Meden Isaac; Ursula Simonis

doi:10.1117/12.823023

14 July 2009 Synthesis and cellular localization of porphyrinic pigments

Sarah Sareh, Sarah Kong, Lenin Parrales, Anna Jung, Kara Cross, Beate Röder, Meden Isaac, Ursula Simonis

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73804R (2009) https://doi.org/10.1117/12.823023
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

To determine factors that govern the uptake preference of photosensitizers in cellular organelles of human adenocarcinoma cells, diarginyl-dialkoxy- and diarginyl-dimethoxyphenylporphyrins (TPPs) and two of their corresponding indium(III) complexes were synthesized, characterized and incubated in androgen-sensitive human prostate adenocarcinoma cells LNCaP. The porphyrins revealed properties that are of importance for phototherapy. They are water-soluble, have their fourth Q-band absorbing at ≈ 650 nm, are taken up in relatively high concentrations in LNCaP cells, and are phototoxic. Colocalization and phototoxicity studies revealed that all porphyrins localized preferentially to the lysosomes and invoked cell death when excited with 650 nm light. Compared to the corresponding methoxy-substituted TPPs, the diargininyl-dialkoxy-substituted porphyrins localized to a small extent in the mitochondria. The corresponding In(III) chloride complexes that are slightly less water-soluble were also taken up in the lysosomes of LnCaP cells. When the TPPs were compared to a pheophorbide derivative recently synthesized in our laboratory, it was determined that the TPPs have a preference for lysosomal localization, whereas the pheophorbide derivative co-localized to the mitochondria. Phototoxicity studies revealed that the longer chain dialkoxyTPPs were more effective in cell killing and induced greater morphological changes typical of apoptotic cell death than the shorter chain methoxy substituted porphyrins. The In(III) complexes seemed to be the most phototoxic. These results highlight that the type, nature, and substitution pattern of the chromophore modulate the extent of apoptotic cell death and influence cellular targeting.

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Sarah Sareh, Sarah Kong, Lenin Parrales, Anna Jung, Kara Cross, Beate Röder, Meden Isaac, and Ursula Simonis "Synthesis and cellular localization of porphyrinic pigments", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73804R (14 July 2009); https://doi.org/10.1117/12.823023

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KEYWORDS

Content addressable memory

Cell death

Magnesium

Cancer

Photodynamic therapy

Chromatography

Luminescence

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