A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

Michael S. D. Smith; Alex C. T. Ko; Andrew Ridsdale; Bernie Schattka; Adrian Pegoraro; Mark D. Hewko; Masashi Shiomi; Albert Stolow; Michael G. Sowa

doi:10.1117/12.840500

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4 August 2009 A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

Michael S. D. Smith, Alex C. T. Ko, Andrew Ridsdale, Bernie Schattka, Adrian Pegoraro, Mark D. Hewko, Masashi Shiomi, Albert Stolow, Michael G. Sowa

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Proceedings Volume 7386, Photonics North 2009; 73860I (2009) https://doi.org/10.1117/12.840500
Event: Photonics North 2009, 2009, Quebec, Canada

Abstract

In this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content.

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Michael S. D. Smith, Alex C. T. Ko, Andrew Ridsdale, Bernie Schattka, Adrian Pegoraro, Mark D. Hewko, Masashi Shiomi, Albert Stolow, and Michael G. Sowa "A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions", Proc. SPIE 7386, Photonics North 2009, 73860I (4 August 2009); https://doi.org/10.1117/12.840500

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