High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

Anders E. Wallin; Heikki Ojala; Gabija Ziedaite; Linda Degerth; Dennis Bamford; Edward Haeggström

doi:10.1117/12.826470

20 August 2009 High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

Anders E. Wallin, Heikki Ojala, Gabija Ziedaite, Linda Degerth, Dennis Bamford, Edward Haeggström

Proceedings Volume 7400, Optical Trapping and Optical Micromanipulation VI; 740007 (2009) https://doi.org/10.1117/12.826470
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.

Citation Download Citation

Anders E. Wallin, Heikki Ojala, Gabija Ziedaite, Linda Degerth, Dennis Bamford, and Edward Haeggström "High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors", Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 740007 (20 August 2009); https://doi.org/10.1117/12.826470

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