Depth of field extension in a low power microscope objective

Pantazis Mouroulis; Byron E. van Gorp; Holly A. Bender; Eric E. Bloemhof; Julia Nichols; Susanne Douglas

doi:10.1117/12.827393

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21 August 2009 Depth of field extension in a low power microscope objective

Pantazis Mouroulis, Byron E. van Gorp, Holly A. Bender, Eric E. Bloemhof, Julia Nichols, Susanne Douglas

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Proceedings Volume 7429, Novel Optical Systems Design and Optimization XII; 742904 (2009) https://doi.org/10.1117/12.827393
Event: SPIE Optical Engineering + Applications, 2009, San Diego, California, United States

Abstract

Three different techniques for extending the depth of field of a low-power (4x) microscope objective system are examined experimentally: wavefront coding with a cubic phase mask, amplitude modulation with a large central obscuration, and added spherical aberration. Their relative merits are discussed and demonstrated with sample images.

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Pantazis Mouroulis, Byron E. van Gorp, Holly A. Bender, Eric E. Bloemhof, Julia Nichols, and Susanne Douglas "Depth of field extension in a low power microscope objective", Proc. SPIE 7429, Novel Optical Systems Design and Optimization XII, 742904 (21 August 2009); https://doi.org/10.1117/12.827393

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