Analog mean delay method (AMD) for real-time fluorescence lifetime microscopy (FLIM)

Sucbei Moon; Youngjae Won; Dug Young Kim

doi:10.1117/12.848242

25 November 2009 Analog mean delay method (AMD) for real-time fluorescence lifetime microscopy (FLIM)

Sucbei Moon, Youngjae Won, Dug Young Kim

Proceedings Volume 7506, 2009 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments; 750605 (2009) https://doi.org/10.1117/12.848242
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

We present a new high-speed lifetime measurement scheme of analog mean-delay (AMD) method which is suitable for studying dynamical time-resolved spectroscopy and high-speed fluorescence lifetime imaging microscopy (FLIM). In our lifetime measurement method, the time-domain intensity of a decaying fluorescence light source is acquired as an analog waveform, and the lifetime information of the source is extracted from the calculated mean temporal delay of the waveform.

Citation Download Citation

Sucbei Moon, Youngjae Won, and Dug Young Kim "Analog mean delay method (AMD) for real-time fluorescence lifetime microscopy (FLIM)", Proc. SPIE 7506, 2009 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, 750605 (25 November 2009); https://doi.org/10.1117/12.848242

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available