PDT-treated apoptotic cells induce macrophage synthesis NO

S. Song; D. Xing; F. F. Zhou; W. R. Chen

doi:10.1117/12.838105

2 December 2009 PDT-treated apoptotic cells induce macrophage synthesis NO

S. Song, D. Xing, F. F. Zhou, W. R. Chen

Proceedings Volume 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging; 75070F (2009) https://doi.org/10.1117/12.838105
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

Nitric oxide (NO) is a biologically active molecule which has multi-functional in different species. As a second messenger and neurotransmitter, NO is not only an important regulatory factor between cells' information transmission, but also an important messenger in cell-mediated immunity and cytotoxicity. On the other side, NO is involving in some diseases' pathological process. In pathological conditions, the macrophages are activated to produce a large quantity of nitric oxide synthase (iNOS), which can use L-arginine to produce an excessive amount of NO, thereby killing bacteria, viruses, parasites, fungi, tumor cells, as well as in other series of the immune process. In this paper, photofrin-based photodynamic therapy (PDT) was used to treat EMT6 mammary tumors in vitro to induce apoptotic cells, and then co-incubation both apoptotic cells and macrophages, which could activate macrophage to induce a series of cytotoxic factors, especially NO. This, in turn, utilizes macrophages to activate a cytotoxic response towards neighboring tumor cells. These results provided a new idea for us to further study the immunological mechanism involved in damaging effects of PDT, also revealed the important function of the immune effect of apoptotic cells in PDT.

Citation Download Citation

S. Song, D. Xing, F. F. Zhou, and W. R. Chen "PDT-treated apoptotic cells induce macrophage synthesis NO", Proc. SPIE 7507, 2009 International Conference on Optical Instruments and Technology: Optical Trapping and Microscopic Imaging, 75070F (2 December 2009); https://doi.org/10.1117/12.838105

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