bFGF immunochemical detection based on surface plasmon resonance

Ying Li; Jingang Zhong; Yonglin Zhang; Yong Tang

doi:10.1117/12.836050

23 November 2009 bFGF immunochemical detection based on surface plasmon resonance

Ying Li, Jingang Zhong, Yonglin Zhang, Yong Tang

Proceedings Volume 7508, 2009 International Conference on Optical Instruments and Technology: Advanced Sensor Technologies and Applications; 75080P (2009) https://doi.org/10.1117/12.836050
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

Surface Plasmon Resonance (SPR) is very sensitive to the change of the refractive index of the dielectric medium on metallic films. Without labeling and purification the samples can quickly, real-timely and dynamically analyze bio-molecular interactions. The biochip detection system based on SPR has been established to detect the immunoreactions for the prepared bFGF protein chip, including the reaction between bFGF antigen and antibody, between bFGF antibody and antibody's antibody respectively. After the immunoreactions occur on sample arrays the refractive index as well as the resonant angle will increase. The reflected light images of the chips taken by the CCD camera can determine the happening of the immunoreactions on the probes of the chips and the existing of substances that are needed to be detected. SPR system can analyze qualitatively and quantitatively.

Citation Download Citation

Ying Li, Jingang Zhong, Yonglin Zhang, and Yong Tang "bFGF immunochemical detection based on surface plasmon resonance", Proc. SPIE 7508, 2009 International Conference on Optical Instruments and Technology: Advanced Sensor Technologies and Applications, 75080P (23 November 2009); https://doi.org/10.1117/12.836050

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