Determining the optimal imaging position in tomographic interference microscopy

Liang Xue; Jian-cheng Lai; Zhen-hua Li

doi:10.1117/12.837106

24 November 2009 Determining the optimal imaging position in tomographic interference microscopy

Liang Xue, Jian-cheng Lai, Zhen-hua Li

Proceedings Volume 7513, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Process Technology; 75130Z (2009) https://doi.org/10.1117/12.837106
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

Tomographic interference microscopy is a method which can obtain the three-dimensional refractive index of live cells and tissues. In this paper, the cone-shaped light with the transverse scanning is adopted, which offers non-contact, highresolution and real-time cell magnifying imaging facilities relatively to the traditional parallel beam projecting on the tissue sample. However, the index-induced focal shift is the common disadvantage for this tomographic interference microscopy, which leads to the decline in image quality and impacts later reconstruction. Then the image sharpness metric based upon the histogram of image to determine the index-induced focal shift is introduced. The experimental results show that the variances of histograms are compatible with a Gaussian function. The peak value of the Gaussian curve corresponds to the optimal imaging position's histogram variance.

Citation Download Citation

Liang Xue, Jian-cheng Lai, and Zhen-hua Li "Determining the optimal imaging position in tomographic interference microscopy", Proc. SPIE 7513, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Process Technology, 75130Z (24 November 2009); https://doi.org/10.1117/12.837106

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