Research on microscopy automatic focusing based on gradient function

Tengfei Zhang; Zexin Xiao; Ran Xiao

doi:10.1117/12.838106

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24 November 2009 Research on microscopy automatic focusing based on gradient function

Tengfei Zhang, Zexin Xiao, Ran Xiao

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Proceedings Volume 7513, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Process Technology; 75132B (2009) https://doi.org/10.1117/12.838106
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

In the digital imaging system, how to achieve automatic focusing rapidly and efficiently is one of key researches on imaging system. The stepping motor subdivision technique is introduced into automatic focusing system in this paper, and quick large range focusing strategy with combination of coarse tuning and trimming is presented, applying focusing system to high power objective lens of more minimal depth of field, further enlarging the application range of automatic focusing. Finally after experimental verification, the requirements of large range, high precision, quick focusing are met in the system which has wide application.

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Tengfei Zhang, Zexin Xiao, and Ran Xiao "Research on microscopy automatic focusing based on gradient function", Proc. SPIE 7513, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Imaging and Process Technology, 75132B (24 November 2009); https://doi.org/10.1117/12.838106

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