Immobilization of human papillomavirus DNA probe for surface plasmon resonance imaging

Xinyuan Chong; Yanhong Ji; Suihua Ma; Le Liu; Zhiyi Liu; Yao Li; Yonghong He; Jihua Guo

doi:10.1117/12.843886

28 October 2009 Immobilization of human papillomavirus DNA probe for surface plasmon resonance imaging

Xinyuan Chong, Yanhong Ji, Suihua Ma, Le Liu, Zhiyi Liu, Yao Li, Yonghong He, Jihua Guo

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75190O (2009) https://doi.org/10.1117/12.843886
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

Human papillomavirus (HPV) is a kind of double-stranded DNA virus whose subspecies have diversity. Near 40 kinds of subspecies can invade reproductive organ and cause some high risk disease, such as cervical carcinoma. In order to detect the type of the subspecies of the HPV DNA, we used the parallel scan spectral surface plasmon resonance (SPR) imaging technique, which is a novel type of two- dimensional bio-sensing method based on surface plasmon resonance and is proposed in our previous work, to study the immobilization of the HPV DNA probes on the gold film. In the experiment, four kinds of the subspecies of the HPV DNA (HPV16, HPV18, HPV31, HPV58) probes are fixed on one gold film, and incubate in the constant temperature condition to get a HPV DNA probe microarray. We use the parallel scan spectral SPR imaging system to detect the reflective indices of the HPV DNA subspecies probes. The benefits of this new approach are high sensitive, label-free, strong specificity and high through-put.

Citation Download Citation

Xinyuan Chong, Yanhong Ji, Suihua Ma, Le Liu, Zhiyi Liu, Yao Li, Yonghong He, and Jihua Guo "Immobilization of human papillomavirus DNA probe for surface plasmon resonance imaging", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190O (28 October 2009); https://doi.org/10.1117/12.843886

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