Single molecular image of cytosolic free Ca2+ of skeletal muscle cells in rats pre- and post-exercise-induced fatigue

Yi Liu; Heming Zhang; Yanping Zhao; Zhiming Liu

doi:10.1117/12.843464

28 October 2009 Single molecular image of cytosolic free Ca²⁺ of skeletal muscle cells in rats pre- and post-exercise-induced fatigue

Yi Liu, Heming Zhang, Yanping Zhao, Zhiming Liu

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Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75191K (2009) https://doi.org/10.1117/12.843464
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

A growing body of literature indicated the cytosolic free Ca²⁺ concentration of skeletal muscle cells changes significantly during exercise-induced fatigue. But it is confusing whether cytosolic free Ca²⁺ concentration increase or decrease. Furthermore, current researches mainly adopt muscle tissue homogenate as experiment material, but the studies based on cellular and subcellular level is seldom. This study is aimed to establish rat skeletal muscle cell model of exercise-induced fatigue, and confirm the change of cytosolic free Ca²⁺ concentration of skeletal muscle cells in rats preand post- exercise-induced fatigue. In this research, six male Wistar rats were randomly divided into two groups: control group (n=3) and exercise-induced fatigue group (n=3). The former group were allowed to freely move and the latter were forced to loaded swimming to exhaustive. Three days later, all the rats were sacrificed, the muscle tissue from the same site of skeletal muscle were taken out and digested to cells. After primary culture of the two kinds of skeletal muscle cells from tissue, a fluorescent dye-Fluo-3 AM was used to label the cytosolic free Ca²⁺. The fluorescent of Ca²⁺ was recorded by confocal laser scanning microscopy. The results indicated that, the Ca²⁺ fluorescence intensity of cells from the rat of exercise-induced fatigue group was significantly higher than those in control group. In conclusion, cytosolic free Ca²⁺ concentration of skeletal muscle cells has a close relation with exercise-induced fatigue, and the increase of cytosolic free Ca²⁺ concentration may be one of the important factors of exercise-induced fatigue.

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Yi Liu, Heming Zhang, Yanping Zhao, and Zhiming Liu "Single molecular image of cytosolic free Ca²⁺ of skeletal muscle cells in rats pre- and post-exercise-induced fatigue", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191K (28 October 2009); https://doi.org/10.1117/12.843464

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KEYWORDS

Luminescence

Calcium

Tissues

Confocal microscopy

Confocal laser scanning microscopy

Glasses

In vitro testing

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