Multimodal confocal mosaicing microscopy: an emphasis on squamous cell carcinoma

Nathaniel W. Chen; Jordan Sensibaugh; Ardaland Ardeshiri; Adam Blanchard; Steven Jacques; Daniel Gareau

doi:10.1117/12.842746

2 March 2010 Multimodal confocal mosaicing microscopy: an emphasis on squamous cell carcinoma

Nathaniel W. Chen, Jordan Sensibaugh, Ardaland Ardeshiri, Adam Blanchard, Steven Jacques, Daniel Gareau

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75480K (2010) https://doi.org/10.1117/12.842746
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Our previous study reported a sensitivity of 96.6% and a specificity of 89.2% in rapidly detecting Basal Cell Carcinomas (BCCs) when nuclei were stained with acridine orange. Squamous Cell Carcinomas (SCCs) and infiltrative BCCs remain difficult to detect. More complete screening can be achieved utilizing both acridine orange for nuclei staining and eosin for cytoplasmic contrast, using two lasers to excite the two stains independently. Nuclear fluorescence is achieved by staining with acridine orange (0.5mM, 60 s), and cytoplasmic fluorescence is achieved by staining with eosin working solution (30 s). This work shows good morphological contrast of SCC and infiltrative BCC with eosin, acridine orange, and reflectance, and presents a means for rapid SCC and infiltrative BCC detection in fresh skin excisions using multimodal confocal microscopy. In addition, digital staining is shown to effectively simulate hematoxylin and eosin (H&E) histology with confocal mosaics.

Citation Download Citation

Nathaniel W. Chen, Jordan Sensibaugh, Ardaland Ardeshiri, Adam Blanchard, Steven Jacques, and Daniel Gareau "Multimodal confocal mosaicing microscopy: an emphasis on squamous cell carcinoma", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480K (2 March 2010); https://doi.org/10.1117/12.842746

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KEYWORDS

Confocal microscopy

Luminescence

Reflectivity

Skin

Collagen

Microscopy

Tumors

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