Raman spectroscopic evidence of crystalline phosphate precursor to bone apatitic mineral

John-David P. McElderry; Guisheng Zhao; Qian Yang; Gurjit Mandair; Renny T. Franceschi; Michael D. Morris

doi:10.1117/12.842497

2 March 2010 Raman spectroscopic evidence of crystalline phosphate precursor to bone apatitic mineral

John-David P. McElderry, Guisheng Zhao, Qian Yang, Gurjit Mandair, Renny T. Franceschi, Michael D. Morris

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 754849 (2010) https://doi.org/10.1117/12.842497
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Bone is a highly specialized connective tissue comprised of cross-linked collagen fibers interspersed with apatitic mineral crystallites of various sizes, shapes, orientation, and composition. However, the nucleation, growth, and propagation of mineral crystallite into the collagenous matrix are not clearly understood. By using a research grade inverted microscope fitted with a line-shaped 830 nm laser and spectrograph, we show that the Raman scatter from mineralizing cell cultures in an incubation chamber can be collected and monitored directly through the bottom of the well-plates over a period of 24 hours. In our studies, murine-derived MC3T3 cells were incubated at 37°C in the presence of 5% CO₂ and 85% humidity. Results show a gradual shift in the phosphate ν₁ apatitic band center (955-957 cm^-1) during the first hour of mineralization. The phosphate ν₁ apatitic band width also narrowed during this time. To quantify the amount of crystal growth in vivo, we used a calibration curve derived from X-ray powder diffraction and Raman studies performed on a series of synthetic carbonated apatites and deproteinated mouse femoral specimens. Mineralization in neonatal mouse calvarial culture was observed along the lambdoid suture. Deposition proceeded in a stepwise fashion over the course of ~30 h.

Citation Download Citation

John-David P. McElderry, Guisheng Zhao, Qian Yang, Gurjit Mandair, Renny T. Franceschi, and Michael D. Morris "Raman spectroscopic evidence of crystalline phosphate precursor to bone apatitic mineral", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 754849 (2 March 2010); https://doi.org/10.1117/12.842497

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