Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds

Darcy S. Peterka; Volodymyr Nikolenko; Elodie Fino; Roberto Araya; Roberto Etchenique; Rafael Yuste

doi:10.1117/12.842606

2 March 2010 Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds

Darcy S. Peterka, Volodymyr Nikolenko, Elodie Fino, Roberto Araya, Roberto Etchenique, Rafael Yuste

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75484P (2010) https://doi.org/10.1117/12.842606
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We have developed a spatial light modulator (SLM) based microscope that uses diffraction to shape the incoming two-photon laser source to any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision at high frame rates. Additionally, we have combined this microscope with a new class of two photon active neuromodulators with Ruthenium BiPyridine (RuBi) based cages that offer great flexibility for neuronal control.

Citation Download Citation

Darcy S. Peterka, Volodymyr Nikolenko, Elodie Fino, Roberto Araya, Roberto Etchenique, and Rafael Yuste "Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75484P (2 March 2010); https://doi.org/10.1117/12.842606

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