Quantitative analysis of thermally-induced alterations of corneal stroma by second-harmonic generation imaging

P. Matteini; F. Rossi; F. Ratto; R. Cicchi; D. Kapsokalyvas; F. S. Pavone; R. Pini

doi:10.1117/12.840674

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2 March 2010 Quantitative analysis of thermally-induced alterations of corneal stroma by second-harmonic generation imaging

P. Matteini, F. Rossi, F. Ratto, R. Cicchi, D. Kapsokalyvas, F. S. Pavone, R. Pini

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Proceedings Volume 7550, Ophthalmic Technologies XX; 755022 (2010) https://doi.org/10.1117/12.840674
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Thermal modifications induced in the corneal stroma were investigated by means of second harmonic generation (SHG) imaging. Whole fresh cornea samples were heated in a water bath at temperatures in the 35-80 °C range for a 4-min time. SHG images of the structural modifications induced at each temperature were acquired from different areas of cross-sectioned corneal stroma by using an 880 nm linearly- and circularly-polarized excitation light emitted by a mode-locked Ti:Sapphire laser. The SHG images were then analyzed by means of both an empirical approach and a 2D-theoretical model. The proposed analyses provide a detailed description of the changes occurring in the structural architecture of the cornea during the thermal treatment. Our results allow us to depict a temperature-dependent biochemical model for the progressive destructuration occurring to collagen fibrils and nonfibrillar components of the stroma.

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P. Matteini, F. Rossi, F. Ratto, R. Cicchi, D. Kapsokalyvas, F. S. Pavone, and R. Pini "Quantitative analysis of thermally-induced alterations of corneal stroma by second-harmonic generation imaging", Proc. SPIE 7550, Ophthalmic Technologies XX, 755022 (2 March 2010); https://doi.org/10.1117/12.840674

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