Availability of fluorescence spectroscopic in the accompaniment of formation of corneal cross-linking

M. M. Costa; C. Kurachi; V. S. Bagnato; S. J. Faria e Sousa; L. Ventura

doi:10.1117/12.842940

2 March 2010 Availability of fluorescence spectroscopic in the accompaniment of formation of corneal cross-linking

M. M. Costa, C. Kurachi, V. S. Bagnato, S. J. Faria e Sousa, L. Ventura

Author Affiliations +

Proceedings Volume 7550, Ophthalmic Technologies XX; 755023 (2010) https://doi.org/10.1117/12.842940
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The corneal cross-linking is a method that associates riboflavin and ultraviolet light to induce a larger mechanical resistance at cornea. This method has been used for the treatment of Keratoconus. Since cross-linking is recent as treatment, there is a need to verify the effectiveness of the method. Therefore, the viability of the fluorescence spectroscopy technique to follow the cross-linking formation at cornea was studied. Corneas were divided in two measuring procedures: M1 (cornea + riboflavin), and M2 (cornea + riboflavina + light irradiation, 365nm). For fluorescence measurements, a spectrofluorimeter was used, where several wavelengths were selected (between 320nm and 370nm) for cornea excitation. Several fluorescence spectra were collected, at 10 min-interval, during 60 min. Spectra allowed one to observe two very well defined bands of fluorescence: the first one at 400nm (collagen), and the second one at 520nm (riboflavin). After spectra analyses, a decrease of collagen fluorescence was observed for both groups. For riboflavin, on the other hand, there was a fluorescence increase for M1, and a decrease for M2. Thus, it is possible to conclude that it this technique is sensitive for the detection of tissue structural changes during cross-linking treatment, encouraging subsequent studies on quantification of cross-linking promotion in tissue.

Citation Download Citation

M. M. Costa, C. Kurachi, V. S. Bagnato, S. J. Faria e Sousa, and L. Ventura "Availability of fluorescence spectroscopic in the accompaniment of formation of corneal cross-linking", Proc. SPIE 7550, Ophthalmic Technologies XX, 755023 (2 March 2010); https://doi.org/10.1117/12.842940

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KEYWORDS

Cornea

Luminescence

Tissues

Collagen

Fluorescence spectroscopy

Optical fibers

Spectroscopy

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