You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
25 February 2010Prevention of bloodstream infections by photodynamic inactivation of multiresistant Pseudomonas aeruginosa in burn wounds
Bloodstream infections are potentially life-threatening diseases. They can cause serious secondary infections, and may
result in endocarditis, severe sepsis or toxic-shock syndrome. Pseudomonas aeruginosa is an opportunistic pathogen and
one of the most important etiological factors responsible for nosocomial infections, mainly in immuno-compromissed
hosts, characteristic of patients with severe burns. Its multiresistance to antibiotics produces many therapeutic problems,
and for this reason, the development of an alternative method to antibiotic therapy is needed. Photodynamic inactivation
(PDI) may be an effective and alternative therapeutic option to prevent bloodstream infections in patients with severe
burns. In this study we report the use of PDI to prevent bloodstream infections in mice with third-degree burns. Burns
were produced on the back of the animals and they were infected with 109 cfu/mL of multi-resistant (MR) P. aeruginosa.
Fifteen animals were divided into 3 groups: control, PDT blue and PDT red. PDT was performed thirty minutes after
bacterial inoculation using 10μM HB:La+3 and a light-emitting diode (LED) emitting at λ=460nm±20nm and a LED
emitting at λ=645 nm±10nm for 120s. Blood of mice were colected at 7h, 10h, 15h, 18h and 22h pos-infection (p.i.) for
bacterial counting. Control group presented 1×104 cfu/mL in bloodstream at 7h p.i. increasing to 1×106 at 22h, while
mice PDT-treated did not present any bacteria at 7h; only at 22h p.i. they presented 1×104cfu/mL. These results suggest
that HB:La+3 associated to blue LED or red LED is effective to delay and diminish MR P.aeruginosa bloodstream
invasion in third-degree-burned mice.
The alert did not successfully save. Please try again later.
M. C. E. Hashimoto, R. A. Prates, D. J. Toffoli, L. C. Courrol, M. S. Ribeiro, "Prevention of bloodstream infections by photodynamic inactivation of multiresistant Pseudomonas aeruginosa in burn wounds," Proc. SPIE 7552, Mechanisms for Low-Light Therapy V, 75520I (25 February 2010); https://doi.org/10.1117/12.840278