Prevention of bloodstream infections by photodynamic inactivation of multiresistant Pseudomonas aeruginosa in burn wounds

M. C. E. Hashimoto; R. A. Prates; D. J. Toffoli; L. C. Courrol; M. S. Ribeiro

doi:10.1117/12.840278

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25 February 2010 Prevention of bloodstream infections by photodynamic inactivation of multiresistant Pseudomonas aeruginosa in burn wounds

M. C. E. Hashimoto, R. A. Prates, D. J. Toffoli, L. C. Courrol, M. S. Ribeiro

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Proceedings Volume 7552, Mechanisms for Low-Light Therapy V; 75520I (2010) https://doi.org/10.1117/12.840278
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Bloodstream infections are potentially life-threatening diseases. They can cause serious secondary infections, and may result in endocarditis, severe sepsis or toxic-shock syndrome. Pseudomonas aeruginosa is an opportunistic pathogen and one of the most important etiological factors responsible for nosocomial infections, mainly in immuno-compromissed hosts, characteristic of patients with severe burns. Its multiresistance to antibiotics produces many therapeutic problems, and for this reason, the development of an alternative method to antibiotic therapy is needed. Photodynamic inactivation (PDI) may be an effective and alternative therapeutic option to prevent bloodstream infections in patients with severe burns. In this study we report the use of PDI to prevent bloodstream infections in mice with third-degree burns. Burns were produced on the back of the animals and they were infected with 10⁹ cfu/mL of multi-resistant (MR) P. aeruginosa. Fifteen animals were divided into 3 groups: control, PDT blue and PDT red. PDT was performed thirty minutes after bacterial inoculation using 10μM HB:La⁺³ and a light-emitting diode (LED) emitting at λ=460nm±20nm and a LED emitting at λ=645 nm±10nm for 120s. Blood of mice were colected at 7h, 10h, 15h, 18h and 22h pos-infection (p.i.) for bacterial counting. Control group presented 1×10⁴ cfu/mL in bloodstream at 7h p.i. increasing to 1×10⁶ at 22h, while mice PDT-treated did not present any bacteria at 7h; only at 22h p.i. they presented 1×10⁴cfu/mL. These results suggest that HB:La⁺³ associated to blue LED or red LED is effective to delay and diminish MR P.aeruginosa bloodstream invasion in third-degree-burned mice.

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M. C. E. Hashimoto, R. A. Prates, D. J. Toffoli, L. C. Courrol, and M. S. Ribeiro "Prevention of bloodstream infections by photodynamic inactivation of multiresistant Pseudomonas aeruginosa in burn wounds", Proc. SPIE 7552, Mechanisms for Low-Light Therapy V, 75520I (25 February 2010); https://doi.org/10.1117/12.840278

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