Mutant and wild type cell chemotaxis in 3D and 4D with ultrahigh-resolution optical coherence tomography

Sara M. Rey; Boris Považay; Bernd Hofer; Angelika Unterhuber; Adrian Harwood; Wolfgang Drexler

doi:10.1117/12.843244

23 February 2010 Mutant and wild type cell chemotaxis in 3D and 4D with ultrahigh- resolution optical coherence tomography

Sara M. Rey, Boris Považay, Bernd Hofer, Angelika Unterhuber, Adrian Harwood, Wolfgang Drexler

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Proceedings Volume 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV; 75540N (2010) https://doi.org/10.1117/12.843244
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Conventionally, cell chemotaxis is studied on two-dimensional (2D) transparent surfaces due to limitations in optical and image data-collection techniques. However, substrates which more closely mimic the natural environment of cells are often opaque or three-dimensional (3D). The non-invasive label-free imaging technique of frequency domain optical coherence tomography (OCT) has high axial and transverse resolution of >4μm, comparatively high penetration depth and the ability to acquire volumes in a few seconds, therefore offering the potential to visualize moving cells in 3D (2D+time) and 4D (3D+time). Cell migration is demonstrated in 3D on opaque surfaces, and in 4D within an agarose gel. The speed and directionality of wild type (Ax2) cell movement is seen to be comparable on agar and nitrocellulose filter substrates. Differences can be clearly seen in the character of cell movement between Ax2, myosin knockout (mhcA-) and PLC null ~10μm Dictyostelium discoideum cells in 4D using ultrahigh resolution OCT. OCT is therefore shown to be a useful technique for the study of cell migration.

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Sara M. Rey, Boris Považay, Bernd Hofer, Angelika Unterhuber, Adrian Harwood, and Wolfgang Drexler "Mutant and wild type cell chemotaxis in 3D and 4D with ultrahigh- resolution optical coherence tomography", Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 75540N (23 February 2010); https://doi.org/10.1117/12.843244

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KEYWORDS

Optical coherence tomography

Video

3D image processing

Opacity

Photonic integrated circuits

Image filtering

Image resolution

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