5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin

Karsten König; Martin Weinigel; Hans George Breunig; Axel Gregory; Peter Fischer; Marcel Kellner-Höfer; Rainer Bückle; Martin Schwarz; Iris Riemann; Frank Stracke; Volker Huck; Christian Gorzelanny; Stefan W. Schneider

doi:10.1117/12.841861

22 February 2010 5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin

Karsten König, Martin Weinigel, Hans George Breunig, Axel Gregory, Peter Fischer, Marcel Kellner-Höfer, Rainer Bückle, Martin Schwarz, Iris Riemann, Frank Stracke, Volker Huck, Christian Gorzelanny, Stefan W. Schneider

Author Affiliations +

Proceedings Volume 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII; 75551I (2010) https://doi.org/10.1117/12.841861
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Some years ago, CE-marked clinical multiphoton systems for 3D imaging of human skin with subcellular resolution have been launched. These tomographs provide optical biopsies with submicron resolution based on two-photon excited autofluorescence (NAD(P)H, flavoproteins, keratin, elastin, melanin, porphyrins) and second harmonic generation by collagen. The 3D tomograph was now transferred into a 5D imaging system by the additional detection of the emission spectrum and the fluorescence lifetime based on spatially and spectrally resolved time-resolved single photon counting. The novel 5D intravital tomograph (5D-IVT) was employed for the early detection of atopic dermatitis and the analysis of treatment effects.

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Karsten König, Martin Weinigel, Hans George Breunig, Axel Gregory, Peter Fischer, Marcel Kellner-Höfer, Rainer Bückle, Martin Schwarz, Iris Riemann, Frank Stracke, Volker Huck, Christian Gorzelanny, and Stefan W. Schneider "5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin", Proc. SPIE 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII, 75551I (22 February 2010); https://doi.org/10.1117/12.841861

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