Quantitative estimation of IL-6 in serum/plasma samples using a rapid and cost-effective fiber optic dip-probe

Chun-Wei Wang; Upender Manne; Vishnu B. Reddy; Rakesh Kapoor

doi:10.1117/12.843807

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24 February 2010 Quantitative estimation of IL-6 in serum/plasma samples using a rapid and cost-effective fiber optic dip-probe

Chun-Wei Wang, Upender Manne, Vishnu B. Reddy, Rakesh Kapoor

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Proceedings Volume 7559, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications X; 75590G (2010) https://doi.org/10.1117/12.843807
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A rapid and cost-effective combination tapered fiber-optic biosensor (CTFOB) dip-probe was used for quantitative estimation of interleukin (IL)-6 in serum/plasma samples. Sandwich immunoassay was used as the detection technique. Probes could successfully detect presence of IL-6 in two serum samples, non-neoplastic autoimmune patient (lupus) sample and lymphoma patient sample. The estimated amount of IL-6 in lupus patient sample was 4.8 ± 0.9 pM and in lymphoma patient sample was 2 ± 1 pM. It is demonstrated that the developed CTFOB dip-probe is capable of quantitative estimation of proteins in serum/plasma samples with high specificity.

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Chun-Wei Wang, Upender Manne, Vishnu B. Reddy, and Rakesh Kapoor "Quantitative estimation of IL-6 in serum/plasma samples using a rapid and cost-effective fiber optic dip-probe", Proc. SPIE 7559, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications X, 75590G (24 February 2010); https://doi.org/10.1117/12.843807

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