Label free investigation of biomolecules on the nanometer scale using tip-enhanced Raman spectroscopy

Volker Deckert; Tanja Deckert-Gaudig; Marc Richter; Regina Treffer; Xiumei Lin

doi:10.1117/12.851078

11 February 2010 Label free investigation of biomolecules on the nanometer scale using tip-enhanced Raman spectroscopy

Volker Deckert, Tanja Deckert-Gaudig, Marc Richter, Regina Treffer, Xiumei Lin

Proceedings Volume 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry; 75600J (2010) https://doi.org/10.1117/12.851078
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In this contribution we present results of tip-enhanced Raman spectroscopy (TERS) measurements on a single crystal of cystine. The observed spectral features lead to the conclusion that the S-S bond was cleaved due to interactions with the silver tip. The spectra differ strongly depending on the site of the molecule interacting with the silver tip. Additionally, first TERS spectra bovine serum albumin (BSA) indicate, that the disulfide bridges of cystine in the protein remain unchanged. This fact can be used for a structural discussion of the secondary structure of the protein. A thorough band assignment was feasible based on an extensive collection of previously obtained TERS spectra of selected amino acids.

Citation Download Citation

Volker Deckert, Tanja Deckert-Gaudig, Marc Richter, Regina Treffer, and Xiumei Lin "Label free investigation of biomolecules on the nanometer scale using tip-enhanced Raman spectroscopy", Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 75600J (11 February 2010); https://doi.org/10.1117/12.851078

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