In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system

Todd N. Erpelding; Chulhong Kim; Manojit Pramanik; Zijian Guo; John Dean; Ladislav Jankovic; Konstantin Maslov; Lihong V. Wang

doi:10.1117/12.841858

23 February 2010 In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system

Todd N. Erpelding, Chulhong Kim, Manojit Pramanik, Zijian Guo, John Dean, Ladislav Jankovic, Konstantin Maslov, Lihong V. Wang

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 756418 (2010) https://doi.org/10.1117/12.841858
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Sentinel lymph node biopsy (SLNB) has become the standard method for axillary staging in breast cancer patients, relying on invasive identification of sentinel lymph nodes (SLNs) following injection of blue dye and radioactive tracers. While SLNB achieves a low false negative rate (5-10%), it is an invasive procedure requiring ionizing radiation. As an alternative to SLNB, ultrasound-guided fine needle aspiration biopsy has been tested clinically. However, ultrasound alone is unable to accurately identify which lymph nodes are sentinel. Therefore, a non-ionizing and noninvasive detection method for accurate SLN mapping is needed. In this study, we successfully imaged methylene blue dye accumulation in vivo in rat axillary lymph nodes using a Phillips iU22 ultrasound imaging system adapted for photoacoustic imaging with an Nd:YAG pumped, tunable dye laser. Photoacoustic images of rat SLNs clearly identify methylene blue dye accumulation within minutes following intradermal dye injection and co-registered photoacoustic/ultrasound images illustrate lymph node position relative to surrounding anatomy. To investigate clinical translation, the imaging depth was extended up to 2.5 cm by adding chicken breast tissue on top of the rat skin surface. These results raise confidence that photoacoustic imaging can be used clinically for accurate, noninvasive SLN mapping.

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Todd N. Erpelding, Chulhong Kim, Manojit Pramanik, Zijian Guo, John Dean, Ladislav Jankovic, Konstantin Maslov, and Lihong V. Wang "In vivo photoacoustic and ultrasonic mapping of rat sentinel lymph nodes with a modified commercial ultrasound imaging system", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756418 (23 February 2010); https://doi.org/10.1117/12.841858

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