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25 February 2010 Label-free quantitative detection of biomarkers
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Abstract
A highly sensitive technique for detection and quantification of protein-analyte binding interaction using spectral domain low coherence interferometric technique is implemented. Using this technique, it is possible to study real time interactions by quantifying the changes in optical path length caused by protein-analyte binding. The technique does not require elaborate sensor preparation and calibration. Any coverslip with certain thickness and capability to bind protein can work using this technique. For proof of principle, the interaction between IgG antigen and anti-IgG was studied and quantification of the accumulation of anti-IgG on sensor surface was demonstrated. Additionally, for biological relevance interactions between cancer biomarker EGFr and EGF was also studied and the preliminary results are presented. The potential applications of the label free technique include point-of-care screening of cancer biomarkers and tumor cell adhesion analysis.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Digant P. Davé and Sajal Chirvi "Label-free quantitative detection of biomarkers", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680Z (25 February 2010); https://doi.org/10.1117/12.842879
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