Two-photon microscopy with dynamic focusing objective using a liquid lens

Kye-Sung Lee; Philip Vanderwall; Jannick P. Rolland

doi:10.1117/12.842581

26 February 2010 Two-photon microscopy with dynamic focusing objective using a liquid lens

Kye-Sung Lee, Philip Vanderwall, Jannick P. Rolland

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756923 (2010) https://doi.org/10.1117/12.842581
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A variable focus microscope without moving parts for axial focusing within the sample in two-photon microscopy can provide a faster, more robust, and cost-effective solution to 3D imaging at high resolution. A custom dynamic focusing objective was recently developed that incorporates a liquid lens within the optical design of a custom microscope. In this paper, we investigate the use of a liquid lens embedded in an aberration-corrected custom microscope for z-dimension scanning in 3D resolved two-photon microscopy. Results show that we can achieve imaging speed of 50 frames per sec for an arbitrary plane in 3D space, a leap forward in scan speed for two-photon microscopy.

Citation Download Citation

Kye-Sung Lee, Philip Vanderwall, and Jannick P. Rolland "Two-photon microscopy with dynamic focusing objective using a liquid lens", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756923 (26 February 2010); https://doi.org/10.1117/12.842581

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