Discrimination of collagen in normal and pathological dermis through polarization second harmonic generation

Ping-Jung Su; Wei-Liang Chen; Jin-Bon Hong; Tsung-Hsien Li; Ruei-Jr Wu; Chen-Kuan Chou; Sung-Jan Lin; Chen-Yuan Dong

doi:10.1117/12.843370

26 February 2010 Discrimination of collagen in normal and pathological dermis through polarization second harmonic generation

Ping-Jung Su, Wei-Liang Chen, Jin-Bon Hong, Tsung-Hsien Li, Ruei-Jr Wu, Chen-Kuan Chou, Sung-Jan Lin, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692A (2010) https://doi.org/10.1117/12.843370
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We used polarization-resolved, second harmonic generation (P-SHG) microscopy at single pixel resolution for medical diagnosis of pathological skin dermis, and found that P-SHG can be used to distinguish normal and dermal pathological conditions of keloid, morphea, and dermal elastolysis. We find that the histograms of the d33/d31 ratio for the pathological skins to contain two peak values and to be wider than that of the normal case, suggesting that the pathological dermal collagen fibers tend to be more structurally heterogeneous. Our work demonstrates that pixel-resolved, second-order susceptibility microscopy is effective for detecting heterogeneity in spatial distribution of collagen fibers.

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Ping-Jung Su, Wei-Liang Chen, Jin-Bon Hong, Tsung-Hsien Li, Ruei-Jr Wu, Chen-Kuan Chou, Sung-Jan Lin, and Chen-Yuan Dong "Discrimination of collagen in normal and pathological dermis through polarization second harmonic generation", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692A (26 February 2010); https://doi.org/10.1117/12.843370

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