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24 February 2010 Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging
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Abstract
Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. We propose here to study long-time duration imaging on different type of adherent cells: green monkey kidney COS7 cells, human breast epithelial MCF10A cells, and human breast cancer derived from MDA-231 cells. This study permits a enhanced visualization of the whole cell life at different levels of confluence. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details and thresholding.
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Pierre Bon, Benoit Wattellier, Serge Monneret, and Didier Marguet "Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700E (24 February 2010); https://doi.org/10.1117/12.844930
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