Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays

D. Geißler; D. Hill; H.-G. Löhmannsröben; E. Thomas; A. Lavigne; B. Darbouret; E. Bois; L. J. Charbonnière; R. F. Ziessel; N. Hildebrandt

doi:10.1117/12.842731

26 February 2010 Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays

D. Geißler, D. Hill, H.-G. Löhmannsröben, E. Thomas, A. Lavigne, B. Darbouret, E. Bois, L. J. Charbonnière, R. F. Ziessel, N. Hildebrandt

Author Affiliations +

Proceedings Volume 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics; 75720C (2010) https://doi.org/10.1117/12.842731
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.

Citation Download Citation

D. Geißler, D. Hill, H.-G. Löhmannsröben, E. Thomas, A. Lavigne, B. Darbouret, E. Bois, L. J. Charbonnière, R. F. Ziessel, and N. Hildebrandt "Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays", Proc. SPIE 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics, 75720C (26 February 2010); https://doi.org/10.1117/12.842731

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