Multi-wavelength spectroscopy of oriented erythrocytes

Yulia M. Serebrennikova; Luis H. Garcia-Rubio; Jennifer M. Smith; Debra E. Huffman

doi:10.1117/12.842046

25 February 2010 Multi-wavelength spectroscopy of oriented erythrocytes

Yulia M. Serebrennikova, Luis H. Garcia-Rubio, Jennifer M. Smith, Debra E. Huffman

Proceedings Volume 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics; 75720E (2010) https://doi.org/10.1117/12.842046
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Accurate characterization of the optical properties of erythrocytes is essential for the applications in optical biomedicine, in particular, for diagnosis of blood related diseases. The observed optical properties strongly depend on the erythrocyte's size, hemoglobin composition and orientation relative to the incident light. We explored the effect of orientation on the absorption and scattering properties of erythrocytes suspended in saline using UV-visible spectroscopy and theoretical predictive modeling based on anomalous diffraction approximation. We demonstrate that the orientation of erythrocytes in dilute saline suspensions is not random and produces consistent spectral pattern. Numerical analysis showed that the multi-wavelength absorption and scattering properties of erythrocytes in dilute suspensions can be accurately described with two orientation populations. These orientation populations with respect to the incident light are face-on incidence and edge-on incidence. The variances of the orientation angles for each population are less than 15 degrees and the relative proportions of the two populations strongly depend on the number density of the erythrocytes in suspensions. Further, the identified orientation populations exhibit different sensitivities to the changes in the compositional and morphological properties of erythrocytes. The anomalous diffraction model based on these orientation populations predicts the absorption and scattering properties of erythrocytes with accuracy greater than 99%. Establishment of the optical properties of normal erythrocytes allows for detection of the disease induced changes in the erythrocyte spectral signatures.

Citation Download Citation

Yulia M. Serebrennikova, Luis H. Garcia-Rubio, Jennifer M. Smith, and Debra E. Huffman "Multi-wavelength spectroscopy of oriented erythrocytes", Proc. SPIE 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics, 75720E (25 February 2010); https://doi.org/10.1117/12.842046

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available