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17 February 2010 Quantum-dot-based quantitative identification of pathogens in complex mixture
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Abstract
In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-, and 100-mer target DNA with both probes was analyzed and quantified by flow cytometry and fluorescence microscopy on the scale of single particles. The following steps were used in the assay: (i) target selection by MP probes and (ii) target detection by QD probes. Hybridization efficiency between MP conjugated probes and target DNA hybrids was controlled by a fluorescent dye specific for nucleic acids. Fluorescence was detected by flow cytometry to distinguish differences in oligo sequences as short as 25-mer capturing in target DNA and by gel-electrophoresis in the case of QD probes. This report shows that effective manipulation and control of micro- and nanoparticles in hybridization assays is possible.
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Sun Hee Lim, Felix Bestwater, Philippe Buchy, Sek Mardy, and Alexey Dan Chin Yu "Quantum-dot-based quantitative identification of pathogens in complex mixture", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750M (17 February 2010); https://doi.org/10.1117/12.851050
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