Cellular uptake of polymeric nanocapsules loaded with ICG by human blood monocytes and human spleen macrophages

Baharak Bahmani; Bongsu Jung; Sharad Gupta; Bahman Anvari

doi:10.1117/12.842354

1 March 2010 Cellular uptake of polymeric nanocapsules loaded with ICG by human blood monocytes and human spleen macrophages

Baharak Bahmani, Bongsu Jung, Sharad Gupta, Bahman Anvari

Author Affiliations +

Proceedings Volume 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II; 75761Q (2010) https://doi.org/10.1117/12.842354
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Indocyanine green (ICG) is an FDA approved near infrared dye used in assessment of hepatic function and ophthalmological vascular imaging. However, given the rapid clearance of ICG from the blood stream, its imaging and phototherapeutic applications remain very limited. As a potential method to increase circulation time of ICG, and extend its clinical applications, we have encapsulated ICG within polymeric based nanoconstructs whose surface can be coated with various materials including polyethylene glycol (PEG). To gain an understanding of the interaction between ICG-containing nanocapsules (ICG-NCs) and vascular cells, we are characterizing the uptake of the nanocapsules coated with various materials by human peripheral blood monocytes and human spleen macrophages using fluorescence microscopy. Results of these studies will be useful in identifying the appropriate coating material that will result in increased circulation time of ICG-NCs within the vasculature.

Citation Download Citation

Baharak Bahmani, Bongsu Jung, Sharad Gupta, and Bahman Anvari "Cellular uptake of polymeric nanocapsules loaded with ICG by human blood monocytes and human spleen macrophages", Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 75761Q (1 March 2010); https://doi.org/10.1117/12.842354

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