Design and fabrication of a PDMS microchip based immunoassay

Guocheng Shao; Wanjun Wang; Jun Wang; Yuehe Lin

doi:10.1117/12.847138

17 February 2010 Design and fabrication of a PDMS microchip based immunoassay

Guocheng Shao, Wanjun Wang, Jun Wang, Yuehe Lin

Proceedings Volume 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII; 75930Q (2010) https://doi.org/10.1117/12.847138
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

In this paper, we describe the design and fabrication of a polydimethylsiloxane (PDMS) microchip for on-chip multiplex immunoassay applications. The microchip consists of a PDMS microfluidic channel layer and a micro pneumatic valve control layer. By selectively pressurizing the pneumatic microvalves, immuno reagents were controlled to flow and react in certain fluidic channel sites. Cross contamination was prevented by tightly closed valves. Our design was proposed to utilize PDMS micro channel surface as the solid phase immunoassay substrate and simultaneously detect four targets antigens on chip. Experimental results show that 20psi valve pressure is sufficient to tightly close a 200μm wide micro channel with flow rate up to 20μl/min.

Citation Download Citation

Guocheng Shao, Wanjun Wang, Jun Wang, and Yuehe Lin "Design and fabrication of a PDMS microchip based immunoassay", Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 75930Q (17 February 2010); https://doi.org/10.1117/12.847138

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