High-throughput single-molecule fluorescence spectroscopy using parallel detection

X. Michalet; R. A. Colyer; G. Scalia; T. Kim; Moran Levi; Daniel Aharoni; Adrian Cheng; F. Guerrieri; Katsushi Arisaka; Jacques Millaud; I. Rech; D. Resnati; S. Marangoni; A. Gulinatti; M. Ghioni; S. Tisa; F. Zappa; S. Cova; S. Weiss

doi:10.1117/12.846784

22 January 2010 High-throughput single-molecule fluorescence spectroscopy using parallel detection

X. Michalet, R. A. Colyer, G. Scalia, T. Kim, Moran Levi, Daniel Aharoni, Adrian Cheng, F. Guerrieri, Katsushi Arisaka, Jacques Millaud, I. Rech, D. Resnati, S. Marangoni, A. Gulinatti, M. Ghioni, S. Tisa, F. Zappa, S. Cova, S. Weiss

Author Affiliations +

Proceedings Volume 7608, Quantum Sensing and Nanophotonic Devices VII; 76082D (2010) https://doi.org/10.1117/12.846784
Event: SPIE OPTO, 2010, San Francisco, California, United States

Abstract

Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept of this technique is to excite and collect light from a very small volume (typically femtoliter) and work in a concentration regime resulting in rare burst-like events corresponding to the transit of a single-molecule. Those events are accumulated over time to achieve proper statistical accuracy. Therefore the advantage of extreme sensitivity is somewhat counterbalanced by a very long acquisition time. One way to speed up data acquisition is parallelization. Here we will discuss a general approach to address this issue, using a multispot excitation and detection geometry that can accommodate different types of novel highly-parallel detector arrays. We will illustrate the potential of this approach with fluorescence correlation spectroscopy (FCS) and single-molecule fluorescence measurements obtained with different novel multipixel single-photon counting detectors.

Citation Download Citation

X. Michalet, R. A. Colyer, G. Scalia, T. Kim, Moran Levi, Daniel Aharoni, Adrian Cheng, F. Guerrieri, Katsushi Arisaka, Jacques Millaud, I. Rech, D. Resnati, S. Marangoni, A. Gulinatti, M. Ghioni, S. Tisa, F. Zappa, S. Cova, and S. Weiss "High-throughput single-molecule fluorescence spectroscopy using parallel detection", Proc. SPIE 7608, Quantum Sensing and Nanophotonic Devices VII, 76082D (22 January 2010); https://doi.org/10.1117/12.846784

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